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Primer sequences for quantitative polymerase chain reaction analysis.
| Primer (5′-3′) | ||
|---|---|---|
| Gene | Forward | Reverse |
| TNF-α | AACTCCAGGCGG | TCCAGCTGCTCCT |
| TGCCTATG | CCACTTG | |
| IL-1 | AGCTTCAGGCAG | TCATCTCGGAGCC |
| GCAGTATC | TGTAGTG | |
| IL-6 | AAGTCCGGAGAG | TGGATGGTCTTGG |
| GAGACTTC | TCCTTAG | |
| IL-10 | ACTCTTCACCTGC | GCTATGCTGCCTGC |
| TCCACTG | TCTTAC | |
| β-actin | CACGAAACTACCT | CATACTCCTGCTTG |
| TCAACTCC | CTGATC | |
TNF, tumor necrosis factor; IL, interleukin.
