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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Chem Res Toxicol. 2017 Aug 2;30(8):1622–1628. doi: 10.1021/acs.chemrestox.7b00127

Figure 3.

Figure 3

Comet assay with 293T cells. 293T cells in culture were treated with DHT (10 µM), TD (10 µM), or DMSO for 3 (DMSO vs DHT *P=0.0268) or 12 h (DMSO vs DHT *P=0.0224, DMSO vs TD *P=0.0262, DHT vs TD *P=0.016). Experiments ae done in duplicates with error bars representing ±SD. A) Cells showing presence of comet with the inset picture representing a magnified image showing comet formation in one cell. B) Quantitation of DNA cleavage measured as the amount of DNA in the tail of the comets formed. The comet image of menadione is shown in the supporting information (Figure S2).