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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Chem Res Toxicol. 2017 Aug 2;30(8):1622–1628. doi: 10.1021/acs.chemrestox.7b00127

Figure 4.

Figure 4

Detection of ROS in HEK293T and HUVEC cells. Cells in culture were either treated with DHT (10 µM) for 1.5 and 3 h (HUVEC 0hr vs 3hr *P=0.0039, 0hr vs menadione *P=0.029 and HEK293T 0hr vs menadione *P=0.020) or menadione (200 µM) for 2 h. Presence of ROS was detected using the deep red CellRox reagent having tetramethylrhodamine (TRITC) as the fluorescent dye. Experiments are done in triplicates with error bars representing ±SEM. A) Microscopic images of cells showing formation of ROS B) Quantitation of ROS measured as the fluorescence intensity within the cell.