Fig. 1.
(A) Overview of the L-lysine biosynthetic pathway in Synechococcus sp. strain PCC 7002. Insert shows a schematic representation of the integration and induction systems of the heterologous genes introduced into the listed genomic loci. Dashed arrows indicate the presence of allosteric regulation of the native enzymes. (B) Verification of chromosomal segregation of heterologous genes. Gels show bands corresponding to PCR products created from colonies of each strain using primers flanking each loci. The length of each expected band is listed in the inset of panel A.