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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Metab Eng. 2017 Oct 28;44:273–283. doi: 10.1016/j.ymben.2017.10.010

Table 2.

Overview of lysine producing strains of Synechococcus sp. strain PCC 7002

Strain Genotype T (°C) Media [aTc] (ng ml−1) Linear Growth Rate (±SD) (gDCW L−1 h−1) Productivity (±SE) (mmol lysine h−1) %Carbon to Lysine (±SE) Titer (±SD) (mM lysine)
A2542 A1838 A2842
AM.319 ybjE 37 A+ - 0.027 ± 0.001 0.004 ± 0.002 2 ± 0.8 0.78 ± 0.13
TK.032 ybjE AK 37 A+ 10 0.021 ± 0.001 0.010 ± 0.001 7 ± 0.8 1.19 ± 0.14
TK.032 ybjE AK 37 A+ 100 0.025 ± 0.001 0.010 ± 0.002 6 ± 1.2 1.25 ± 0.12
TK.032 ybjE AK 37 A+ 1000 0.023 ± 0.002 0.019 ± 0.002 12 ± 1.1 1.74 ± 0.12
TK.032 ybjE AK 37 A+ 1000 0.020 ± 0.002 0.021 ± 0.002 15 ± 0.0 1.67 ± 0.15
TK.031 ybjE DHDPS 37 A+ 10 0.021 ± 0.001 0.007 ± 0.000 5 ± 0.3 0.67 ± 0.05
TK.031 ybjE DHDPS 37 A+ 100 0.022 ± 0.001 0.012 ± 0.001 8 ± 0.1 1.07 ± 0.16
TK.031 ybjE DHDPS 37 A+ 1000 0.019 ± 0.001 0.011 ± 0.001 8 ± 0.7 1.15 ± 0.31
TK.033 ybjE AK DHDPS 37 A+ 10 0.019 ± 0.001 0.008 ± 0.001 6 ± 0.9 0.80 ± 0.20
TK.033 ybjE AK DHDPS 37 A+ 100 0.023 ± 0.001 0.010 ± 0.001 6 ± 0.3 0.80 ± 0.20
TK.033 ybjE AK DHDPS 37 A+ 1000 0.016 ± 0.002 0.014 ± 0.002 12 ± 0.1 1.02 ± 0.11
TK.032 ybjE AK 37 A+ 1000 0.018 ± 0.002 0.019 ± 0.003 15 ± 0.1 1.33 ± 0.18
TK.036 ybjE PEPC 37 A+ 10 0.022 ± 0.002 0.009 ± 0.002 6 ± 0.9 0.61 ± 0.12
TK.036 ybjE PEPC 37 A+ 100 0.020 ± 0.002 0.009 ± 0.001 6 ± 0.4 0.58 ± 0.03
TK.036 ybjE PEPC 37 A+ 1000 0.018 ± 0.001 0.008 ± 0.001 5 ± 0.9 0.55 ± 0.03
TK.032 ybjE AK 37 A+ w/24 mM NO3 100 0.013 ± 0.001 0.006 ± 0.001 7 ± 0.4 1.14 ± 0.46
TK.032a ybjE AK 37 A+ w/24 mM NH4 100 0.017 ± 0.001 0.020 ± 0.002 18 ± 0.0 3.11 ± 0.22
TK.032b ybjE AK 27 GBF 1000 0.014 ± 0.002 0.003 ± 0.000 3 ± 0.3 0.28 ± 0.06
TK.032 ybjE AK 27 A+ 1000 0 nd nd nd

Note: All experiments done with 1% (v/v) CO2 and 200 μmol photons m−2s−1 and induction with 1 mM IPTG.

Blocked rows report data from experiments run at the same time.

a

pH was manually adjusted to ≈ 8 using NaOH daily

b

Gravity Belt Filtrate (GBF) was diluted to 12.5% (v/v) using secondary effluent both obtained from the Nine Springs Wastewater Treatment Plant (Dane County, Wisconsin, USA)