Table 3.
Chromosome aberration assay and relative cell counts of Gyejibokryeong-hwan aqueous extract (GJBRHE)
| Nominal conc. of GJBRHE (μg/mL) | S9 mix | Time hours a) | Mean total aberrant metaphases | Mean total aberrations | Mean of PP + ER | Relative cell counts (%) |
|---|---|---|---|---|---|---|
| 6 h treatment (+S9) | ||||||
| 0 | + | 6–18 | 1.0/0.5b) | 1.0/0.5 | 0.0 + 0.0 | 100 |
| 1050 # | + | 6–18 | 0.5/0.5 | 0.5/0.5 | 1.0 + 0.0 | 98 |
| 2100 #§ | + | 6–18 | 1.5/0.5 | 1.5/0.5 | 0.0 + 0.0 | 80 |
| 4200 #§ | + | 6–18 | 21.0/21.0** | 36.0/36.0 | 1.5/0.0 | 47 |
| 4500 #§ | + | 6–18 | Not counted | 43 | ||
| CPA 6 | + | 6–18 | 24.0/24.0** | 38.0/37.5 | 0.5 + 0.0 | 63 |
| 6 h treatment (-S9) | ||||||
| 0 | – | 6–18 | 0.5/0.0 | 0.5/0.0 | 0.0 + 0.0 | 100 |
| 225 | – | 6–18 | Not counted | 94 | ||
| 450 #§ | – | 6–18 | 1.5/1.0 | 1.5/1.0 | 1.5 + 0.0 | 80 |
| 900 #§ | – | 6–18 | 1.0/1.0 | 1.0/1.0 | 0.0 + 0.0 | 66 |
| 1050 #§ | – | 6–18 | 2.0/0.5 | 2.0/0.5 | 1.0 + 0.0 | 50 |
| EMS 800 | – | 6–18 | 25.5/25.0** | 37.0/36.0 | 0.0 + 0.0 | 58 |
| 22 h treatment (-S9) | ||||||
| 0 | – | 22–2 | 1.0/1.0 | 2.0/2.0 | 0.5 + 0.0 | 100 |
| 180 | – | 22–2 | 1.5/1.0 | 1.5/1.0 | 0.0 + 0.0 | 89 |
| 360 #§ | – | 22–2 | 2.5/1.5 | 4.0/2.0 | 0.5 + 0.0 | 79 |
| 720 #§ | – | 22–2 | Not counted | 66 | ||
| 800 #§ | – | 22–2 | 7.5/6.5** | 9.5/7.5 | 1.0 + 0.0 | 48 |
| EMS 600 | – | 22–2 | 43.5/43.0** | 69.5/68.5 | 0.0 + 0.0 | 41 |
#Visible turbidity of test item was observed at the beginning of treatment
§Visible turbidity of test item was observed at the end of the treatment
**Significantly different from the control at P < 0.01
a)Treatment time-recovery time
b)Gaps included/excluded, means of duplicate cultures; 100 metaphases were examined per culture