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Comparison of the energy landscapes of the surface peptides on AuNPs conjugated by the traditional one-anchor-point method and our two-anchor-point method. The conformational space of the peptide by the two-anchor-point method is only a small subset (magenta circle) of that by the one-anchor point method (whole cyan sheet), and can be further reduced to the approximation of the binding conformation (red circle) by adjusting the peptide density on the surface of AuNPs.
