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. 2017 Dec 18;115(1):E34–E43. doi: 10.1073/pnas.1713526115

Fig. 3.

Fig. 3.

Interaction between the anti-lysozyme Goldbody and HEWL. (A) Enzymatic activity assays of 30 nM HEWL alone, in the presence of 24 nM nonfunctionalized AuNPs (3.6 nm), or in the presence of 24 nM AuNPs (3.6 nm) functionalized with 60 Pep1, 60 Pep1m, or 60 Pep 1s per AuNP, respectively. Free Pep1 at the equivalent peptide concentration (24 × 60 = 1,440 nM) was used as the control. (B) Slopes of 30 nM HEWL assay curves in the presence of 24 nM AuNPs (3.6 nm) functionalized with different numbers of Pep1 (red) or Pep1s (gray). The white column denotes the free HEWL control. (C) Slopes of 30 nM HEWL assay curves in the presence of 24 nM different AuNP–20Pep1–xPep1s, showing the effect of peptide density [changed by the number (x) of the nonactive Pep1s]. (D) IC50 of AuNP–Pep1 and Tri-NAG for 30 nM HEWL. Error bars indicate SDs.