Table 2.
The normalized fluorescence values of lectin microarray in DN and NDRD groups
| Lectins | Sugar chain structures | DN (n = 19) | NDRD (n = 18) | ||
|---|---|---|---|---|---|
| DN group I (n = 9) | DN group II (n = 10) | MN (n = 10) | IgAN (n = 8) | ||
| Jacalin | Galβ1-3-GalNAcα-Ser/Thr(T), GalNAcα-Ser/Thr(Tn), GlcNAcβ1-3-GalNAcα-Ser/Thr(Core3), sialyl-T (ST). Not bind to Core2, Core6, and sialyl-Tn (STn) | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.023 ± 0.001 | 0.000 ± 0.000 |
| MAL-II | Siaα-2-3Galβ1-4Glc(NAc)/Glc, Siaα2-3Gal, Siaα2-3, Siaα2-3GalNAc | 0.017 ± 0.001 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| PTL-I | GalNAc, GalNAcα-1,3Gal, GalNAcα-1,3Galβ1,3/4Glc | 0.020 ± 0.001 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| SJA | Terminal in GalNAc and Gal, anti-A and anti-B human blood group | 0.024 ± 0.001 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| AAL | Fucα1-6 GlcNAc (core fucose), Fucα1-3(Galβ1-4) GlcNAc | 0.030 ± 0.006 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.060 ± 0.018 |
| DBA | α-GalNAc, Tn antigen, GalNAcα1-3((Fucα1-2)) Gal (blood group A antigen) | 0.022 ± 0.003 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| RCA120 | β-Gal, Galβ-1,4GlcNAc (type II), Galβ1-3GlcNAc (type I) | 0.084 ± 0.001 | 0.108 ± 0.006 | 0.407 ± 0.002 | 0.064 ± 0.010 |
| STL | Trimers and tetramers of GlcNAc, core (GlcNAc) of N-glycan, oligosaccharide containing GlcNAc and MurNAc | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.042 ± 0.002 | 0.540 ± 0.043 |
| BS-I | α-Gal, α-GalNAc, Galα-1,3Gal, Galα-1,6Glc | 0.033 ± 0.004 | 0.020 ± 0.003 | 0.064 ± 0.005 | 0.000 ± 0.000 |
| ConA | High-Mannose, Manα1-6(Manα1-3) Man, αMannose, αGlc | 0.000 ± 0.000 | 0.012 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| PTL-II | Gal, blood group H, T-antigen | 0.017 ± 0.001 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| DSA | (GlcNAc) 2-4, polyLacNAc and LacNAc (NA3, NA4) | 0.439 ± 0.009 | 0.462 ± 0.017 | 0.221 ± 0.014 | 0.164 ± 0.016 |
| SBA | α- or β-linked terminal GalNAc, (GalNAc) n, GalNAcα1-3Gal, blood-group A | 0.066 ± 0.010 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.022 ± 0.001 |
| PSA | Fucα-1,6GlcNAc, α-D-Man, α-D-Glc | 0.016 ± 0.004 | 0.000 ± 0.000 | 0.033 ± 0.007 | 0.000 ± 0.000 |
| UEA-I | Fucα1-2Galβ1-4Glc (NAc) | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.021 ± 0.003 | 0.000 ± 0.000 |
| PWM | (GlcNAc)n and polyLacNAc | 0.034 ± 0.004 | 0.020 ± 0.002 | 0.054 ± 0.005 | 0.038 ± 0.002 |
| GNA | HighMannose, Manα1-3Man | 0.073 ± 0.006 | 0.048 ± 0.003 | 0.046 ± 0.001 | 0.030 ± 0.003 |
| PHA-E + L | Bisecting GlcNAc, bi-antennary N-glycans, tri- and tetra-antennary complex-type N-glycan | 0.016 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 | 0.000 ± 0.000 |
| SNA | Sia2-6Gal/GalNAc | 0.112 ± 0.017 | 0.328 ± 0.021 | 0.090 ± 0.002 | 0.083 ± 0.015 |
The signal intensities obtained for three biological replications were normalized and represented as mean ± SD. NDRD: Nondiabetic renal disease; DN: Diabetic nephropathy; SD: Standard deviation; MN: Membranous nephropathy; IgAN: IgA nephropathy; DSA: Datura stramonium agglutinin.