Table 2.
Effect Of 4 Weeks Of Exenatide Treatment On The Mrna Levels Of Genes Involved In Lipid Metabolism, and Inflammation In The Liver Of Male Mice
| RD‐S | RD‐Ex | HF‐S | HF‐Ex | |
|---|---|---|---|---|
| Cc1 +/+ | ||||
| Lipid metabolism | ||||
| Srebp‐1c | 0.74 ± 0.07 | 0.79 ± 0.06 | 3.23 ± 0.21† | 0.88 ± 0.05* |
| Fasn | 0.35 ± 0.03 | 0.45 ± 0.03 | 2.61 ± 0.15† | 0.54 ± 0.07* |
| Cd36 | 0.43 ± 0.04 | 0.48 ± 0.03 | 3.25 ± 0.22† | 0.52 ± 0.05* |
| Fatp‐1 | 0.77 ± 0.05 | 0.65 ± 0.03 | 2.04 ± 0.13† | 0.69 ± 0.05* |
| Fatp‐4 | 0.89 ± 0.04 | 0.76 ± 0.06 | 2.62 ± 0.19† | 0.74 ± 0.06* |
| Inflammation | ||||
| F4/80 | 0.79 ± 0.04 | 0.75 ± 0.04 | 2.55 ± 0.17† | 0.80 ± 0.06* |
| Cd68 | 0.87 ± 0.09 | 0.81 ± 0.07 | 3.25 ± 0.20† | 0.99 ± 0.07* |
| Il‐1β | 3.51 ± 0.32 | 3.57 ± 0.30 | 9.07 ± 0.37† | 4.35 ± 0.26* |
| Il‐6 | 4.15 ± 0.24 | 4.57 ± 0.18 | 8.95 ± 0.27† | 4.88 ± 0.28* |
| Ifn‐γ | 6.47 ± 0.20 | 6.59 ± 0.26 | 13.0 ± 0.43† | 6.49 ± 0.25* |
| Tnf‐α | 2.65 ± 0.26 | 2.79 ± 0.25 | 6.70 ± 0.23† | 2.39 ± 0.17* |
| Cc1 –/– | ||||
| Lipid metabolism | ||||
| Srebp‐1c | 1.42 ± 0.16 | 1.65 ± 0.11 | 4.36 ± 0.16† | 3.93 ± 0.15† |
| Fasn | 0.82 ± 0.05 | 0.80 ± 0.05 | 1.53 ± 0.06† | 1.57 ± 0.09† |
| Cd36 | 0.74 ± 0.05 | 0.76 ± 0.03 | 3.25 ± 0.22† | 3.28 ± 0.20† |
| Fatp‐1 | 0.93 ± 0.04 | 0.90 ± 0.04 | 2.75 ± 0.20† | 2.84 ± 0.20† |
| Fatp‐4 | 0.97 ± 0.04 | 0.96 ± 0.04 | 3.42 ± 0.15† | 3.68 ± 0.19† |
| Inflammation | ||||
| F4/80 | 1.93 ± 0.16 | 1.83 ± 0.17 | 4.79 ± 0.21† | 5.08 ± 0.20† |
| Cd68 | 1.34 ± 0.11 | 1.83 ± 0.13 | 5.96 ± 0.27† | 5.86 ± 0.35† |
| Il‐1β | 6.16 ± 0.26 | 6.11 ± 0.26 | 12.4 ± 0.36† | 12.2 ± 0.51† |
| Il‐6 | 6.22 ± 0.24 | 6.57 ± 0.18 | 12.5 ± 0.40† | 12.0 ± 0.38† |
| Ifn‐γ | 8.18 ± 0.25 | 8.39 ± 0.17 | 15.9 ± 0.26† | 16.3 ± 0.30† |
| Tnf‐α | 4.53 ± 0.21 | 4.34 ± 0.18 | 10.0 ± 0.32† | 10.2 ± 0.24† |
Mice (3 months of age) were fed an RD or HF diet for 2 months. During the last month of feeding, mice were given saline or exenatide (20 ng/g BW) once daily by intraperitoneal injections (n = 5/genotype per feeding per treatment). Liver extracts were analyzed by qRT‐PCR normalized to 18S in triplicate. Values are expressed as mean ± SEM; *P < 0.05 Ex versus S/feeding group; † P < 0.05 HF versus RD/treatment group.
Abbreviations: BW, body weight; Cd, clusters of differentiation; Ex, exenatide; Fatp, fatty acid transport protein; Il, interleukin; Ifn, interferon; Tnf, tumor necrosis factor.