FIGURE 2.

Increased total extracellular lactate correlates with increasing proliferation in stimulated T cell cultures. Cell proliferation dye V450–labeled T cells were cultured in the presence and absence of anti-CD3/CD28 Dynabeads for 6 d. At days 1, 4, and 6, cell culture supernatants were sampled for lactate analysis, and cells were analyzed by flow cytometry for proliferation status (cell proliferation dye dilution), as well as counterstained with dead cell marker (Zombie NIR) and Abs to CD69-allophycocyanin and CD25-PE. (A) The flow gating strategy: dead cells, activated cells, proliferating cells, and nonproliferating cells were gated. (B) Correlation of total extracellular lactate concentration with T cell proliferation.