FIGURE 6.
Screening of blood donors for responses to CMV peptides. Data from PBMCs of eight healthy donors, cultured with soluble anti-CD3 or one of two different CMV peptide pools (IE1 and gB; 1 μg/ml). PBMCs were plated at 105 cells per well in triplicates in 96-well plates. (A) IFN-γ ELISPOT was performed at 48 h, and a mean spot-forming unit per million > 100 was taken as a positive response. Extracellular lactate was measured in cell culture supernatants at days 2 and 5. (B) Day-5 positive total lactate concentrations. All four seropositive donors were identified as responders to one or both peptides by both methods.