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. 2017 Dec 5;15(2):1728–1736. doi: 10.3892/ol.2017.7523

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Copyright: © Hecht et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Differential activity of EFV in cancer cells and fibroblasts. BxPC-3 pancreatic cancer cells were compared to SBL-5 primary skin fibroblasts. (A) Effect of EFV treatment for 72 h on apoptitic death (vertical lines) or necrotic death (horizontal lines) analyzed by flow cytometry after Annexin/7AAD staining. Expression of cell cycle regulators Cyclin D1, E2F1 and p27Kip1 after EFV (40 µmol/l) treatment on (B) BxPC-3 and (C) SBL-5 cells. Expression of anti-apoptotic signal BCL 2 and Caspase 8 after EFV (40 µmol/l) treatment on (D) BxPC-3 and (E) SBL-5 cells. (F) Reference genes for the BxPC-3 pancreatic cancer cells were the combination of RNA pseudouridylate synthase domain containing 3, transcription factor 20 and GIGYF2 and for SBL-5 fibroblasts the combination of GIGYF2 and L3MBTL2 polycomb repressive complex 1 subunit. Expression of LINE1-encoded endogenous reverse transcriptase open reading frame 2. Reference gene for the comparison was HPRT1. Example of the spots of the PathScan^® Stress and Apoptosis Signaling Antibody Array in the fluorescence readout for (G) BxPC-3 and (H) SBL-5. *P<0.05. GIGYF2, GRB10 interacting GYF protein 2; EFV, Efavirenz.