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. 2017 Dec 5;15(2):1728–1736. doi: 10.3892/ol.2017.7523

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Copyright: © Hecht et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — EFV-induced oxidative stress. (A) Flow cytometric assessment and (B) immunostaining of oxidative stress induction with DHE in BxPC-3 pancreatic cancer cells and SBL-5 primary fibroblasts treated with EFV (40 µmol/l for 1 h). (C) Time kinetics of oxidative stress induction after EFV treatment (40 µmol/l) in life cell imaging in BxPC-3 cells. Fluorescence intensity was scanned at two positions with EFV (solid lines) and two positions without EFV (dotted lines). (D) Simultaneous flow cytometric assessment of oxidative stress (DHE), mitochondrial membrane potential (DilC) and apoptosis (Annexin V) after EFV treatment. DHE, Dihydroethidium; EFV, Efavirenz.