Fig. 2.

Effects of anthocyanins on intracellular lipid accumulation and TG content in the differentiated 3T3-L1 cells. 3T3-L1 preadipocytes were treated with the indicated concentrations of anthocyanins during differentiation. (A) and (B) on day 8, (A) the cells were directly observed via an inverted microscope, or (B) the cells were fixed and stained with Oil Red O to visualize lipid droplets (Magnification, ×200). (C) The TG content was measured to quantify the intracellular lipid content at 500 nm wavelength, using the ELISA reader. The data were expressed as the mean ± SD of three independent experiments (*p < 0.05, vs. undifferentiated control; ^#p < 0.05, vs. differentiated control).