Fig. 4.

Effects of AMPK inhibitor on the anthocyanins-induced activation of AMPK in the differentiated 3T3-L1 cells. 3T3-L1 cells were pre-treated with compound C, an AMPK inhibitor, for 1 hr, and then treated with 200 μg/mL anthocyanins. On day 8, completely differentiated cells were lysed, and the cellular proteins were separated electrophoretically using SDS-polyacrylamide gels, and transferred onto membranes. The membranes were probed with the indicated antibodies. Proteins were observed using an ECL detection system. Actin was used as an internal control. The relative ratios of the phosphorylation types relative to the total protein amounts in the results of the Western blotting are presented at the bottom. The data were expressed as the mean ± SD of three independent experiments (*p < 0.05, vs. undifferentiated control; ^#p < 0.05, vs. differentiated cells treated with anthocyanins alone).