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Effect of isoliquiritigenin (ISL) on AMPK-SIRT1 pathway in AML-12 cells. (A) Representative images of oil red O staining (×200). (B) Bar graph showing the triglyceride content in AML-12 cells after 3-day treatment with a combination of 200 mM ethanol and 0, 5, 10, 20, or 50 μM ISL. (C) Representative images of western blots showing the effect of 3 days treatment with a combination of 200mM ethanol and 0, 5, 10, 20, or 50 μM ISL on SIRT1-AMPK signaling. ***p < 0.001; ###p<0.001.
