Fig. 6.

Effects of LPS pretreatment on the LPS-induced production of cytokines and CRP in Hepa-1c1c7 cells. Hepa-1c1c7 cells were pretreated with LPS 50 pg/mL in fresh complete media once a day for 2 days and new Hepa-1c1c7 cells were pretreated with LPS 50 ng/mL once on only 2nd day. And then both of LPS-pretreated Hepa-1c1c7 cells were cultured in fresh complete media for 24 hr or 48 hr in the presence of LPS 10 μg/mL. These experiments were run in tetraplicate and repeated at least twice. Each value represents the mean ± SE. *p < 0.05 and **p < 0.01. Significantly different from the value in each positive control. ^#p < 0.05. Significantly different from the value in each SL-LPS.