Figure 3.

Western blot and RT-PCR analyses of p21 (FK506 binding protein like), p27 (cyclin dependent kinase inhibitor 1B) and p53 mRNA and protein expression in 2-mM SB-treated A172 cells. (A) A172 cells were treated with 2 mM SB for the indicated time and the protein levels of the cell cycle regulators p21, p27 and p53 were analyzed by western blot analysis. (B) A172 cells were treated with 2 mM SB for the indicated time and mRNA levels of p21, p27 and p53 were analyzed by RT-PCR. RT-PCR, reverse transcription-polymerase chain reaction; SB, sodium butyrate; bp, base pairs.