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. 2017 Dec 5;15(2):1495–1502. doi: 10.3892/ol.2017.7518

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Copyright: © Nakagawa et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Reversibility of the effects of SB in A172 cells. (A) At 4 days after treatment with 2 mM SB, A172 cells were washed in media without SB and cultured for 4 additional days. The effects on the expression of p21 (FK506 binding protein like), p27 (cyclin dependent kinase inhibitor 1B), p53, SIRT1, pY397/pY577 FAK and p-MLC20 were determined by western blotting. (B) Cell motility was measured in A172 cells treated as in (A). Results are presented as the mean ± standard deviation (n=6). *P<0.01 vs. control. SB, sodium butyrate; SIRT, sirtuin; FAK, focal adhesion kinase; p-, phosphorylated; MLC, myosin light chain.