Table I.
Physical status of HPV 16 and HPV 18 determined by qPCR, ISH and L1 methylation.
| A, HPV 16 physical status, n (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| qPCR (n=100) | ISH (n=140) | L1 methylation (n=154) | |||||||
| Diagnosis | Episomal (n=2) | Mixed (n=52) | Integrated (n=46) | Episomal (n=4) | Mixed (n=66) | Integrated (n=70) | Episomal (n=51) | Mixed (n=91) | Integrated (n=12) |
| Non-IL | 1 (4) | 12 (46) | 13 (50) | 3 (10) | 17 (57) | 10 (33) | 17 (52) | 16 (48) | 0 (6) |
| LSIL | 1 (2) | 27 (59) | 18 (39) | 1 (1) | 43 (54) | 36 (45) | 28 (34) | 48 (59) | 6 (7) |
| HSIL | 0 (0) | 1 (33) | 2 (67) | 0 (0) | 4 (67) | 2 (33) | 4 (45) | 4 (45) | 1 (10) |
| CC | 0 (0) | 12 (48) | 13 (52) | 0 (0) | 2 (8) | 22 (92) | 2 (7) | 23 (77) | 5 (16) |
| B, HPV 18 physical status, n (%) | |||||||||
| qPCR (n=19) | ISH (n=30) | L1 methylation (n=33) | |||||||
| Diagnosis | Episomal (n=5) | Mixed (n=12) | Integrated (n=2) | Episomal (n=1) | Mixed (n=14) | Integrated (n=15) | Episomal (n=0) | Mixed (n=23) | Integrated (n=10) |
| Non-IL | 2 (67) | 1 (33) | 0 (0) | 1 (17) | 5 (83) | 0 (0) | 0 (0) | 4 (67) | 2 (33) |
| LSIL | 1 (10) | 8 (80) | 1 (10) | 0 (0) | 9 (47) | 10 (53) | 0 (0) | 15 (75) | 5 (25) |
| HSIL | 2 (50) | 2 (50) | 0 (0) | 0 (0) | 0 (0) | 2 (100) | 0 (0) | 3 (75) | 1 (25) |
| CC | 0 (0) | 1 (50) | 1 (50) | 0 (0) | 0 (0) | 3 (100) | 0 (0) | 1 (33) | 2 (67) |
HPV, human papillomavirus; qPCR, quantitative polymerase chain reaction; ISH, in situ hybridization; non-IL, non-intraepithelial lesion; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion; CC, cervical carcinoma.