Table II.
Concordance rates of qPCR, ISH and HPV L1 methylation in the detection of the physical status of HPV 16 and HPV 18 in clinical samples.
| A, qPCR/ISH (n=107) | |||
|---|---|---|---|
| qPCR | |||
| Physical status | Mixed/integrated | Episomal | Positive concordance, %a |
| ISH | 96.1 | ||
| Mixed/integrated | 98 | 2 | |
| Episomal | 6 | 1 | |
| B, qPCR/L1 methylation (n=120) | |||
| qPCR | |||
| Physical status | Mixed/integrated | Episomal | Positive concordance, %a |
| L1 methylation | 76.7 | ||
| Mixed/integrated | 74 | 7 | |
| Episomal | 38 | 0 | |
| C, ISH/L1 methylation (n=172) | |||
| ISH | |||
| Physical status | Mixed/integrated | Episomal | Positive concordance, %a |
| L1 methylation | 84.8 | ||
| Mixed/integrated | 123 | 2 | |
| Episomal | 42 | 3 | |
a
Values of 60–80% were considered to indicate good concordance between the techniques and values >80% were considered to indicate very good concordance. HPV, human papillomavirus; qPCR, quantitative PCR; ISH, in situ hybridization.