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. 2017 Dec 5;15(2):1673–1679. doi: 10.3892/ol.2017.7524

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Copyright: © Shen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — MDM4 plays a necessary role during miR-370-mediated cancer cell growth inhibition. (A) Western blotting determination of MDM4 expression in miR-NC and miR-370 transfected LoVo cells treated with or without NSC207895. β-actin was used as loading control. (B) Relative expression analysis of MDM4 expression. n=3, **P<0.01, compared with miR-NC transfected group; ns, no significant difference, compared with miR-NC transfected group with NSC207895 treatment. (C) Analysis of cell viability by CCK-8 kit in miR-NC and miR-370 transfected LoVo cells that treated with or without NSC207895. **P<0.01, compared with miR-NC group. MDM4, mouse double minute 4; miR, microRNA; CCK-8, Cell Counting kit-8.