Table I.
Antibodies used for immunohistochemistry.
| Antigen | Animal | Clone | Source | Processing method | Dilution | Automatic stainer |
|---|---|---|---|---|---|---|
| Ki-67 | Mouse | MIB-1 | DAKO Cytomation, Glostrup, Denmark | HT | 1:100 | Nichirei, Tokyo, Japan |
| CD56 | Mouse | CD564 | Novocastra, Newcastle upon Tyne, UK | HT | 1:50 | Nichirei, Tokyo, Japan |
| Chromogranin A | Rabbit | Poly | DAKO Cytomation, Glostrup, Denmark | HT | 1:500 | Nichirei, Tokyo, Japan |
| CK AE1/AE3 | Mouse | AE1/AE3 | Novocastra, Newcastle upon Tyne, UK, | HT-RTU | 1:100 | Nichirei, Tokyo, Japan |
| NSE | Rabbit | Poly | Nichirei, Tokyo, Japan | Nichirei, Tokyo, Japan |
Method Stain after deparaffinization and activation of the antigen Stainer Histostainer 36A (Nichirei, Tokyo, Japan). CD, cluster of differentiation; CK, cytokeratin; NSE, neuron-specific enolase.