Fig. 4.

Mechanisms that elevate switch probabilities. (A) Illustration of a cargo approaching a normal intersection with a separation of 0 µm. As shown, the cargo is attached to the upper (primary) MT via two motors. The cargo has three options, as labeled: 1, Unengaged motors can bind the primary MT at a site distal to filament intersection (a pass would occur if the proximal motors were to be subsequently released); 2, unengaged motors can bind the crossing MT; or 3, motors engaged on the primary MT can detach, then rebind to the crossing MT. All motors are in green regardless of engagement status. (B) Illustration of a cargo engaged in a ToW at an acute 0.5-µm intersection. A minimal ToW with only two motors is shown for visual clarity. The forces exerted by motors are counteraligned (gray arrows) and serve to “wedge” the cargo between the MTs. Red arrows point to MT plus ends.