Fig. 3.

Quantification of Ca²⁺ response and ICW propagation. (A) Small-region analysis for determining C_ICW. Cells #1 and #2 are the cells shown in Fig. 2A (S_d = 30 µm) and Fig. 2C (S_d = 40 µm), respectively. Insets show the schematics for small-region analysis. (B) C_ICW vs. S_d. The error bars indicate the SD of the calculated averaged uncertainties. (C) Membrane poration and Ca²⁺ response probability at different S_d. (D) Correlations of the maximum intracellular Ca²⁺ concentration change Δ[Ca²⁺]_i(max) (filled symbols) and C_ICW (open symbols) vs. PI uptake in the cytosol at S_d = 30 μm to 50 µm. Results for different S_d are labeled with different symbols and colors. The dashed lines indicate separation of data based on the threshold of PI uptake of 10 (a.u.), and C_ICW of 30 µm/s. (Inset) C_ICW vs. the square root of concentration change rate (Δ[Ca²⁺]_i(max)/t_r)^1/2, where t_r is the risetime from baseline to peak concentration. The dashed line represents a linear fitting of the data (R² = 0.75).