Fig. 5.

Up-regulated Wnt/β-catenin signaling plays a key role in FD. (A) Representative µCT scans of the humerus of littermate control [Gnas^f(R201H)/+] and OsxCre; Gnas^f(R201H)/+ and OsxCre; Gnas^f(R201H)/+; Lrp6^C/+ mutant mice. Sagittal and transverse section views of humerus are also shown. (B) H&E staining of humerus sections from P21 mice of the indicated genotypes. (C) qRT-PCR analysis of humerus bones from mice with the indicated genotypes. (D) Alizarin red staining of BMSCs transduced with Ad-Cre and Ad-GFP viruses 7 d after osteogenic differentiation. At day 0 of osteogenic differentiation, BMSCs were treated with DMSO or LGK-974, a small molecule that inhibits Wnt secretion. (E) Alizarin red staining was quantified. (F and G) qRT-PCR analysis of gene expression in BMSCs at day 7 post differentiation. *P < 0.05, **P < 0.001; ns, not significant; data are presented as mean ± SD.