Fig. 5.

In vivo imaging and anticancer tumor effect of BP@Hydrogel drug delivery platform. (A) Thermal images of mice bearing tumors after injection of DOX or BP@Hydrogel, followed by exposure to 808-nm laser irradiation (1.0 W/cm², 5 min). (Scale bars: 1 cm.) (B) Tumor temperature changes of mice bearing MDA-MB-231 tumors during laser irradiation as indicated in A. (C) Fluorescence images of mice at 1 h, 12 h, and 24 h postirradiation after the in vivo photothermal assay. The 485-nm wavelength light was used as the excitation source and 590 nm was detected as the emitted light. (Scale bars: 1 cm.) (D) Corresponding growth curves of tumors in different groups of mice treated with PBS solution, DOX, BP@Hydrogel depot only, and BP@Hydrogel depot with laser irradiation. The relative tumor volumes were normalized to their initial size. D, Inset shows representative photographs of tumors removed from the killed nude mice. (E) Body weight of nude mice recorded every other day after various treatments. (F) H&E-stained images of major organs from treated mice. (Scale bars: 50 μm.)