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Eukaryotic termination at modified stop codons. (A) Potential hydrogen-bond interactions (dashed lines) between eRF1 (dark gray) and the UAA stop codon (yellow) in a U-turn-like confirmation (37). (B–D) Modified stop codons were incorporated in Flag-eGFP reporter mRNAs and were transfected into HEK293T cells. Twenty-four hours after transfection, the Flag-tagged translation products were detected by Western blotting. LE, long exposure; SE, short exposure.
