Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 4;115(3):619–624. doi: 10.1073/pnas.1715137115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

PMC Copyright notice

Fig. 4. — Effect of evolved BipA OTS on biocontainment strain escape and fitness. (A) Escape frequencies over time for adk.d6 strains transformed with constructs indicated in legend below plots. Green and yellow circles compare escape frequencies of parent and evolved variants and are most relevant for this study. Navy circles represent previously published data (from ref. 3). Gray circles for adk.d6 represent our repeat of previously published data. KA, kanamycin+arabinose; SCA, SDS+chloramphenicol+arabinose. Error bars in A–C represent SEM, n = 3. (B) Escape frequencies over time for tyrS.d8 strains. Lines represent assay detection limit in cases where no colonies were observed. (C) Escape frequencies over time for adk.d6/tyrS.d8 strains. (D) Doubling time for biocontained strains with parental (P) or variant 10 OTS. Error bars = SD, n = 3.