Fig. 2.

(A) A mouse Ephx2 promoter region containing an ∼1,200-bp sequence upstream of the start codon (ATG in red), with 21 CpG sites numbered as −1 ∼ −21 (gray shadow). The primers for MSP are shown with black underline arrows. The primers for both proximal (magenta underline arrows) and distal sequence (green underline arrows), ChIP PCR primers (red underline arrows), and specific binding elements for transcription factors of SP1 (gray box), AP-1 (red box), and NF-κB (blue box) are indicated. (B) Within the diagram of the mouse Ephx2 promoter region, each designated color or font pattern corresponds to the region on the nucleotide sequence in A. Additionally, the position of each GpG site is demarcated by gray bars.