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Binding constants and fit parameters for ubiquitin-binding-domain mutations in ExoU
| Sample | Kd, μM | R2 |
| WT ExoU | 122.8 ± 11.5 | 0.99 |
| T519D | >2,000 | N/A |
| E520R | 420.4 ± 92 | 0.98 |
| T522D | >2,000 | N/A |
| V523D | >2,000 | N/A |
| S527L | 31.9 ± 5 | 0.99 |
| T554D | 92.4 ± 16.7 | 0.99 |
N/A, not available.
