Fig. 6.

Characterization of an RT inhibitor targeting the p51-p66 interface. (A) Three compounds from the NCI DTP library inhibit the fluorescence-labeled RNA/DNA hybrid binding to RT at various concentrations in the presence of Ca²⁺. (B) Structure diagram of NSC73416. Diagrams of the other two compounds are provided in SI Appendix, Fig. S8. (C) NSC73416 inhibits the HIV-1 RNase H with IC₅₀ = 12 µM, but has no effects on human (Hs) or bacterial (Bh) RNase H1. (D) NSC73416 inhibits the HIV-1 polymerase activity with IC₅₀ = 10 µM.