Fig. 7.

The diversity and relative abundance of Hodgkinia lineages in replicate specimens from single populations of (A) Tettigades chilensis (represented by specimen PL301) and (B) T. undata (represented by specimen TETLON), based on amplicon sequencing of rpoB gene. Colors represent different unique rpoB genotypes. The genotypes that are found in only a small proportion of specimens are indicated with arrowheads. Maximum likelihood trees for all genotypes from a population reveal that the genotypes found in a small proportion of specimens differ from widespread genotypes at a single nucleotide position (99.8% identity). They may indicate either single-nucleotide polymorphisms of little functional significance, or ongoing Hodgkinia splits. Preliminary genome data suggest that, in at least some instances, genotypes that differ at a single position do represent recent splitting events.