Fig. 1.

Phylogenetic and population genetic analyses of P. euphratica and P. pruinosa. (A) The biogeographic regions of whole-genome–sequenced individuals for P. euphratica (three lineages identified here: PeC, PeE, and PeW) and P. pruinosa (a single lineage identified here: Pp). The colored areas indicate biogeographic distribution of P. euphratica (gray) and P. pruinosa (pink), and the frequencies of each lineage in potential hybrid and/or backcross populations are shown using larger pie charts. (B) Phylogenetic network inferred using the Neighbor-Net method based on genome-wide SNPs. The ancestral state was identified by genotyping the segregating sites with 10 additional poplar species (see SI Appendix for detailed information). (C and D) PCA plots of SNP data for P. euphratica (+) and P. pruinosa (x). (E) Population structure bar plots. Each vertical bar represents a single individual, and the height of each color represents the probability of assignment to that cluster. The number of individuals in each lineage is also shown.