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. 2017 Dec 26;115(2):391–396. doi: 10.1073/pnas.1714670115

Fig. 4.

Fig. 4.

Hepatic autophagy is important for ammonia detoxification. (A and B) Representative bands and densitometric quantifications of Western blotting of ATG7, p62, and LC3 of livers harvested at 1.0 h post-NH4Cl injection from Atg7fl/fl mice injected with HDAd-Cre or with a control vector (HDAd-AFP) 4 wk before the ammonia challenge (n ≥ 3/group). GAPDH was used as loading control. (C) Serum ammonia at baseline, 15 min and 0.5 h after i.p. injections of NH4Cl in Atg7fl/fl mice injected with HDAd-Cre (n = 5) or HDAd-AFP vector (n = 4). (D) Serum ammonia in WT (Control, n = 12) and TFEB liver-specific knockout mice (TFEB LiKO, n = 13) at baseline, 0.5 and 1.0 h after i.p. injections of NH4Cl. (E) Serum ammonia in WT mice injected with HDAd-TFEB or HDAd-AFP vector (n = 5/group) at baseline, 0.5 and 1.0 h after i.p. injections of NH4Cl. (F and G) Urea and glutamine in livers harvested at 2.0 h post-NH4Cl injection in WT mice injected with HDAd-AFP or HDAd-TFEB (n = 5/group). Control untreated WT mice are included (n = 3). *P < 0.05; **P < 0.01. ns, not significant.