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. 2017 Dec 8;15(2):2296–2304. doi: 10.3892/ol.2017.7571

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Copyright: © Vieira et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Relative ROCK1 and ROCK2 expression in EWS cell lines. (A) Relative mRNA expression levels of ROCK1 and ROCK2 in primary osteoblast cell lines and the EWS cell lines RD-ES and SK-ES-1. ROCK2, but not ROCK1 was found with significantly higher expression (P=0.03) when compared to primary osteoblast cell lines with a 1.97 fold-change for RD-ES and 1.68 for SK-ES-1. Each column represents the mean ± standard deviation. (B) Protein expression levels of ROCK1, ROCK2 and the endogenous GAPDH in SK-ES-1 and RD-ES cell lines. The expression levels of both kinases were found comparable. ROCK, Rho-associated coiled-coil containing protein kinases; EWS, Ewing's sarcoma; FC, fold-change.