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. 2017 Dec 13;15(2):2211–2217. doi: 10.3892/ol.2017.7598

Figure 3.

Figure 3.

FASN regulates glucose metabolism through PKM2 in PDAC cells. (A-C) PANC-1 and MIA PaCa-2 cells were transfected with PKM2 shRNA and negative control. After 48 h, cells were harvested and (A) western blot analysis of the WCL was performed, as well as measurement of (B) glucose consumption and (C) L-Lactate production in the spent medium. Data are presented as the mean ± SD from three replicates. (D) PANC-1 cells were transfected with indicated plasmids and, after 24 h, cells were harvested for western blot analysis. (E and F) Measurement of (E) glucose consumption and (F) L-Lactate production in the spent medium of PANC-1 and MIA PaCa-2 cells 48 h after transfection with FASN shRNA and negative control. Data are presented as the mean ± SD from three replicates. (G and H) Measurement of (G) glucose consumption and (H) L-Lactate production in the spent medium of PANC-1 and MIA PaCa-2 cells 48 h after transfection with the indicated constructs. Data are presented as the mean ± SD from three replicates. *P<0.05 vs. shControl or shFASN+Myc-PKM2 groups. FASN, fatty acid synthase; PKM2, pyruvate kinase M2; shRNA, short hairpin RNA; SD, standard deviation; IB, immunoblot; Myc, V-myc avian myelocytomatosis viral oncogene homolog.