Figure 4.

FASN contributes to gemcitabine resistance. (A) Western blot analysis of whole-cell lysates of PANC-1 and MIA PaCa-2 cells 48 h after transfection with the indicated shRNAs. Cells were treated with or without gemcitabine (10 µM) for 24 h prior to harvest. (B) PANC-1 and MIA PaCa-2 cells were treated as in (A). At 48 h after treatment, caspase-3 activity was measured in the cells. Data are presented as the mean ± SD from two replicates. (C) Cells were treated as in (A). At different time points, cell viability was measured using a MTS Cell Proliferation assay. Data are presented as the mean ± SD from three replicates. *P<0.05. FASN, fatty acid synthase; SD, standard deviation; OD, optical density; IB, immunoblot.