Figure 5.

Cajal band disruption and DRP2 impairment in mutant ipsilateral nerves after injury. A, TEM pictures showing a lack of appositions between SC plasma membrane and the abaxonal layer of the myelin sheath in mutant mice. Scale bars: 2 μm; insert, 1 μm. Asterisk shows Cajal bands location; arrow points to the apposition. B, Quantification. C, Periaxin and DRP2 were quantified by Western blot 1 month after injury in sciatic nerves of MEK1DD mutant mice (MEK) compared with control (Ctr) mice; calnexin was used as a loading control. D, Teased fibers from ipsilateral sciatic nerves showed decreased internodal distance in mutant mice. n = 3 mice/group. *p < 0.05, ***p < 0.001, two-tailed unpaired t test. Holm–Sidak test was performed in B. Error bars indicate SEM.