Figure 2.

Identification and reconstruction of the AIS and somatodendritic domain of dopaminergic neurons. A1–A4, Neurobiotin (Nb)-labeled (A1) and TH-expressing (A2) soma. A distal Nb-labeled process from same neuron (A3, arrow) expresses Ank-G (A4, arrow). A5, As seen in the flattened z-stacked image, this AIS is short and distally located (arrowheads, soma in dashed lines). A6, A caudal view of the 3D reconstruction and inset (black, cell body and dendrites; red, AIS; blue, axon) for the same neuron. B1–B5, Nb-labeled (B1) and TH-expressing soma (B2, dashed lines). A proximal process is indicated (arrows in B1, B2), which expresses Ank-G (B3, arrow). In this case, the AIS is longer and proximally located, as shown in the flattened image (B4) and a caudal view of the 3D reconstruction and inset for the same neuron (B5), color coded as in A6. SNl, Substantia nigra pars lateralis; D, dorsal; R, rostral; L, lateral.