Figure 3.

RB and p-AKT but not PIK3CG expression can be regulated by Notch signaling in pancreatic cancer. (A) Western blot analysis of pan-RB, p-RB and PIK3CG in shLFNG-MIA PaCa-2 and control cells. β-actin was included as loading control. (B) Western blot analysis of p-AKT and pan-AKT in shLFNG-MIA PaCa-2 and control cells. β-actin was included as loading control. (C) Western blot analysis of Notch3, RB and p-RB in MIA PaCa-2 cells overexpressing Notch3IC. β-actin was included as loading control. RB, retinoblastoma-associated protein; p-AKT, phosphorylated RAC-α serine/threonine-protein kinase; PIK3CG, phosphoinositide-3 kinase catalytic subunit-γ; Notch, neurogenic locus notch homolog protein; shLFNG, short hairpin RNA targeted at lunatic fringe; P-, phosphorylated.