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. 2017 Dec 5;15(2):1861–1868. doi: 10.3892/ol.2017.7520

Figure 4.

Figure 4.

Growth inhibition in CRC cells mediated by the knockdown of FAM83H-AS1 was reversed by the Notch1 activator. (A) The expressions of Notch1 and Hes1 at the mRNA and protein levels were detected by western blotting and reverse transcription-quantitative polymerase chain reaction when cells were transfected with sh-FAM83H-AS1. (B) Rescue assays were employed to detect the expressions of Notch1 and Hes1 following the silence of FAM83H-AS1 and the addition of Jagged-1/Fc. (C) MTT and (D) colony formation assays were performed to measure the viability and growth ability of cells treated with sh-FAM83H-AS1 and Jagged-1/Fc. Data are presented as the mean ± standard deviation of at least three independent experiments. Scale bars, 200 µm. *P<0.05 and **P<0.01 vs. control group. CRC, colorectal carcinoma; FAM83H-AS1, FAM83H antisense RNA 1 (head to head); sh, small hairpin; Hes1, Hes family basic-helix-loop-helix transcription factor 1.