Figure 5.

Effect of FAM83H-AS1 on CRC cells was dependent on the Notch signaling pathway. (A) The level of Notch1 was silenced by DAPT in CRC cells based on reverse transcription-quantitative polymerase chain reaction. (B) The effect of silenced Notch1 on the expression level of FAM83H-AS1. (C) Rescue assays were applied to determine the unchanged proliferation of CRC cells following the silencing of Notch1 and FAM83H-AS1 induced by DAPT. Data are presented as the mean ± standard deviation of at least three independent experiments. **P<0.01 vs. DMSO (control) group. DAPT, N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester; CRC, colorectal carcinoma; FAM83H-AS1, FAM83H antisense RNA 1 (head to head); sh, small hairpin.