Figure 2.

NRP-1 knockdown inhibits the viability and activation of LX2 cells. (A) NRP-1 shRNA efficiently knocked down NRP-1, as detected by western blotting; n=3. (B) MTT assay of LX2 optical density in various groups. n=3 repeats. (C) Western blot analysis revealed that the expression levels of α-SMA in LX2-NRP-1 shRNA cells were significantly lower compared with in LX2-NT shRNA cells; n=3. *P<0.05 by analysis of variance. NRP-1, neuropilin-1; shRNA, short hairpin RNA; α-SMA, α-smooth muscle actin; NT, non-targeting; OD, optical density.