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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: Int J Cancer. 2017 Jul 31;141(10):2121–2130. doi: 10.1002/ijc.30893

Figure 2. The effect of TSTs on AR gene expression in CRPC cell lines.

Figure 2

Cells were treated with TST-A at 0, 0.5, 2, 5 nM (A) or TST-D at 0, 5, 20, 50 nM (B) for 4 hours, total cellular RNA and cell lysates were prepared and subjected to western blot and qRT-PCR analyses. The relative AR-V7 mRNA expression from each treatment was normalized with 18S rRNA then calculated using control (=1). Cells were treated with 5 nM TST-A or 50 nM TST-D, cell pellets were collected 0, 2, 4, 8 hours after treatment. Total cellular RNA and cell lysates were prepared and subjected to qRT-PCR analyses (C) or western blot (D).