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22RV1 (A) and VCaP (B) cells were transfected with GFP-vec or GFP-AR-V7 cDNA for 24 hours, cells were subjected to different concentrations of TST-D. For determining cell growth, cells were harvested 24 hours after treatment (upper panel). For analyzing protein expression, cells were collected 4 hours after treatment (lower panel). * P<0.05, ** P<0.01.
