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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: Int J Cancer. 2017 Jul 31;141(10):2121–2130. doi: 10.1002/ijc.30893

Figure 4. The mechanism of TST action on AR gene splicing.

Figure 4

22RV1, VCaP and HEK293 cells were transiently transfected with mock vector or ARV7 minigene plasmid. 24 hours after transfection, cells were treated with TST-A at 0, 0.5, 2, 5 nM (A) or TST-D at 0, 5, 20, 50 nM (B) for 4 h respectively. Relative mRNA levels of AR-V7 mRNA levels to 18S rRNA were determined by real-time qPCR.