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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Immunol. 2017 Mar 1;198(7):2671–2680. doi: 10.4049/jimmunol.1601736

FIGURE 2.

FIGURE 2

KELlo RBCs fail to induce detectable anti-KEL Abs. C57BL/6 recipients negative for the KEL Ag were transfused with KEL (A and B) or KELlo (C and D) RBCs, followed by serological detection for IgM anti-KEL or IgG anti-KEL at the time points indicated. C57BL/6 KEL− recipients were exposed to one, two, or three doses of KEL (E) or KELlo (F) RBCs, followed by examination of IgG anti-KEL Ab formation at day 14 posttransfer. (G) C57BL/6 KEL− recipients were exposed to a normal dose of KEL RBCs or three times the normal dose of KELlo RBCs, followed by evaluation of IgG anti-KEL Abs at day 14 posttransfer. Error bars indicate SEM. *p < 0.05, **p < 0.01, one-way ANOVA.