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. Author manuscript; available in PMC: 2019 Jan 18.
Published in final edited form as: Cell Chem Biol. 2017 Nov 5;25(1):100–109.e8. doi: 10.1016/j.chembiol.2017.09.011

Figure 4.

Figure 4

Relative phosphorylation of OMM PKA-Reporter in response to 1 min light stimulation with different optoPKA constructs, where mCherry (Cry2-mCh) serves as a non-kinase control. optoPKAW196R/Y204A elicits robust phosphorylation of the PKA-Reporter upon illumination (light grey bar) compared to dark (medium grey bar) or (light + H89) (black bar) conditions. All data presented as mean +/- SEM, N ≥ 10 cells per bar. Representative images are found in Figure S3.